Orally delivered animal solutions to benefit humans and the environment
The OrisBio© Oral-Delivery Platform – Delivering Disease Prevention®
Different Species – Different Routes – Different Vaccines
The program begins with an animal target species and disease model. US BIOLOGIC scientists determine the antigens most likely to be effective against the target diseases, and we examine closely the gut and digestive path of the target animal. Each animal species is different, as is its feeding habits, its environments, and its biome.
Field Mouse
- Wild-bred
- Can carry multiple pathogens
- Outdoors
- Large numbers
- Multiple environments
- Uncontrolled eating habits
- Single-stomach digestive track
Canine
- Wild-bred or pure-breed
- Indoor or outdoors
- Multiple sizes / breeds
- Large numbers
- Controlled or uncontrolled eating habits
- Strong preferences for certain foods
- Single-stomach digestive track
Poultry
- Controlled breeding
- Food animal
- Multiple stocks and lifespans (breeder, broiler, layer)
- Large numbers
- Special feed / water requirements (focus on nutrition)
- Single stomach with crop digestive track
Livestock
- Controlled breeding
- Multiple breeds / sizes
- Food animal (meat and milk)
- Long lifespan
- Special feed / water requirements (focus on nutrition)
- Multi-stomached digestive track
Substrate Components
- Allows integration of diverse and species-specific substrates on which vaccines / therapeutics / additives may be incorporated as an amalgam or as a spray-on.
- Enables targeted gut placement while maintaining integrity in harsh GI environments.
- Features the use of coarse textures, providing mechanical stimulation of the animal’s innate immune system.
- Includes Kibble (smaller animal); Chewable treats (large and small animals); Hard chews (e.g., chew bone); Effervescent tablets (for water system application); and Powders (water or feed application).
Bacterial Expression Vehicle Component
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Vehicle inactivation (downstream process, maintains whole-cell, non-GMO, USDA-supported, increased shelf-life).
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Recombinant antigen expression system – AVOIDS use of sub-disease or attenuated infectious agents
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Molecular adjuvantization (cost reduction / no chemical complications).
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Features:
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Gut-microbiome friendly (i.e., prebiotic and probiotic) bacterial vehicles
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Supports complementary antigens, prodrugs, etc.
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Allows active ingredient inclusion
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Chemical Encapsulation Component
- Matrix features encapsulation, allowing flexibility in addressing variant levels of gut pH.
- Matrix serves as a prebiotic.
- Matrix application is high-throughput, low-cost, and resistant to heat.
- Matrix, along with inactivation, allows extended shelf-life.
Manufacturing Features
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Avoids energy-intensive expense of lyophilization.
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Low-cost capital equipment build / compact design.
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Allows distributed manufacturing centers.
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Modular equipment design: Allows coating of a variety of pellet substrates or powdered variants.
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High throughput pellet-coating:
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Large quantity of doses with maintained batch consistency.
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Combined with stabilized inactivation, avoids many challenges associated with cold-chain transport.
Publications
Stafford KC 3rd, Williams SC, van Oosterwijk JG, et al. Field evaluation of a novel oral reservoir-targeted vaccine against Borrelia burgdorferi utilizing an inactivated whole-cell bacterial antigen expression vehicle. Exp Appl Acarol. 2020 Jan 2.
Gomes-Solecki M, Arnaboldi PM, Backenson PB, et al. Protective Immunity and New Vaccines for Lyme Disease. Clin Infect Dis. 2019 Oct 17.
Zatechka S. Reservoir-Targeted Vaccines as a One Health Path to Prevent Zoonotic Disease. Int J Vaccines and Vaccination. 2016;6(2).
Gomes-Solecki M. Blocking pathogen transmission at the source: reservoir targeted OspA-based vaccines against Borrelia burgdorferi. Front Cell Infect Microbiol. 2014 Sep 26;4:136.
Meirelles Richer L, Brisson D, Melo R, et al. Reservoir Targeted Vaccine Against Borrelia burgdorferi: A New Strategy to Prevent Lyme Disease Transmission. J Infect Dis. 2014 Feb 12.
Meirelles Richer L, Aroso M, et al. Reservoir targeted vaccine for Lyme borreliosis induces a yearlong, neutralizing antibody response to OspA in white-footed mice. Clin Vaccine Immunol. 2011 Nov;18(11):1809-16.
Gomes-Solecki MJ, Brisson DR, Dattwyler RJ. Oral vaccine that breaks the transmission cycle of the Lyme disease spirochete can be delivered via bait. Vaccine. 2006 May 15;24(20):4440-9.